The development of plant pathogenic fungi in field is controlled in many ways. Among these, the use of fungicides is widespread. However, moulds have strong adaptation skills that can ultimately reduce the effectiveness of the fungicide used. It appears therefore essential to monitor these changes both in space (on the surface of cultivated land) and time (from year to year). This is the monitoring.
In this context, we put at your disposal our expertise to make your monitoring. You send us samples of leaves (eg monitoring Septoria on wheat) or swabs (eg monitoring Botrytis on grapes). Then we determine in a controlled and standardized test the type of resistance strains by strains of the sampled mould. A report is made by sampling site and by geographic area if applicable. The results obtained are discussed. Data tables are provided in full at the end of the study. We store all of them and we can, if you desired, do a comparison of historical data in correlation with our analysis.
Our method is derived from the germination test. A major effort of standardization has been achieved so the results are comparable both spatially and temporally. This standardization is applied to laboratory test (age of cultures, concentration of spores ...), data processing and analysis. Indeed, measures of elongation of germ tubes are made automatically by image analysis in order to avoid any operators and strains fitness effects. It provides an increase of measurement speed (ability to perform a monitoring more important in a shorter time). All images taken during monitoring are stored. In addition to collect historic reliable data, this technique allows to obtain homogeneous and rigorous monitoring.
When pathogens implicated are uncultivable with in vitro techniques, we can set up a lab test on plant. The development of new test will be done in order to define standardized conditions to obtain reproducible results.
We carry a monitoring on Botrytis cinerea in grapes vines since 2008 on France. We also conducted a monitoring on Septoria tritici of wheat (2009, research TriHR).
We conducted tests of biocides efficacy on Alternaria alternata and solani. We also compared the difference in aggressiveness ofAlternaria alternata and Alternaria solani on potatoes leaf with an in vitro test developed internally. The results of these manipulations have been published in the journal Planet Potato No. 19 (November / December 2009) entitled "Blight of Potato in France".
We have plans to strengthen diagnostics for resistance by combined use of molecular biology with tests already in place.